SIMULASI PLATELET-RICH PLASMA (PRP): PENGARUH KONSENTRASI DAN AKTIVASI TERHADAP MODEL PENYEMBUHAN LUKA

Pravil Mistryanto Tambunan

doi:10.51771/fj.v5i2.1544

Authors

Pravil Mistryanto Tambunan Universitas Tjut Nyak Dhien

DOI:

https://doi.org/10.51771/fj.v5i2.1544

Keywords:

Platelet-Rich Plasma, aktivasi PRP, penyembuhan luka, gel fibrin, simulasi laboratorium

Abstract

Platelet-Rich Plasma (PRP) merupakan salah satu inovasi dalam terapi regeneratif yang memanfaatkan konsentrasi trombosit tinggi untuk mempercepat proses penyembuhan luka. Penelitian ini mensimulasikan pemisahan PRP di laboratorium dan menganalisis efek aktivasi PRP terhadap pembentukan gel fibrin sebagai model penyembuhan luka. Proses sentrifugasi menghasilkan fraksi PRP simulasi dengan konsentrasi protein sebesar 5,2 mg/mL, sedangkan fraksi PPP hanya 2,1 mg/mL, berdasarkan hasil spektrofotometri pada panjang gelombang 280 nm. Aktivasi PRP menggunakan CaCl₂ 10% mempercepat pembentukan gel fibrin, dengan waktu rata-rata koagulasi 3,5 menit, dibandingkan PRP non-aktif (8,7 menit) dan PPP (tidak membentuk gel hingga 15 menit). Hasil ini menegaskan bahwa aktivasi PRP secara signifikan meningkatkan kecepatan dan kualitas pembentukan gel fibrin, yang berperan penting dalam proses regenerasi jaringan. Simulasi ini memberikan pemahaman mendasar mengenai mekanisme kerja PRP, sekaligus menyoroti pentingnya teknik aktivasi dalam optimalisasi manfaat klinis PRP. Secara keseluruhan, penelitian ini membuktikan bahwa konsentrasi protein dan aktivasi PRP merupakan faktor kunci dalam efektivitas terapi regeneratif, dengan simulasi laboratorium ini memberikan model pembelajaran yang aman dan praktis untuk memahami mekanisme dasar PRP. Temuan ini diharapkan dapat menjadi dasar pengembangan praktikum biomedis dan terapi regeneratif berbasis PRP di masa depan.

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