SIMULTANEOUS ANALYSIS OF THIAMINE AND PYRIDOXINE IN TABLETS USING THE UV SPECTROPHOTOMETRIC ABSORPTION FACTOR METHOD

M. Deddy Harfiansyah; Robiatun Rambe; Athaillah Athaillah; Fitria Fitria; Ummi Khairani Rambe

doi:10.51771/fj.v6i02.2256

Authors

M. Deddy Harfiansyah Universitas Haji Sumatera Utara
Robiatun Rambe Universitas Haji Sumatera Utara
Athaillah Athaillah Universitas Haji Sumatera Utara
Fitria Fitria Universitas Ubudiyah Indonesia
Ummi Khairani Rambe Universitas Haji Sumatera Utara

DOI:

https://doi.org/10.51771/fj.v6i02.2256

Keywords:

UV spectrophotometric, absorption factor, thiamine, pyridoxine

Abstract

Thiamine and pyridoxine are frequently combined in a single neurotropic tablet, yet their overlapping UV absorption spectra complicate simultaneous quantification, and a simplified absorption factor procedure dedicated to this specific binary combination has not been established. A UV spectrophotometric method based on the absorption factor approach was developed for the simultaneous quantification of thiamine and pyridoxine in tablet dosage forms using methanol as the solvent system. The proposed method was intended to minimize spectral overlap between both vitamins through mathematical absorbance correction at selected wavelengths. Thiamine showed maximum absorption at 239 nm, whereas pyridoxine exhibited its absorption maximum at 291 nm. Calibration curves demonstrated satisfactory linear relationships within the evaluated concentration ranges. Method validation was carried out according to ICH recommendations by assessing linearity, limit of detection, limit of quantification, accuracy, and precision. The percentage recovery values obtained for thiamine and pyridoxine were 98.48% and 100.43%, respectively, while the percentage relative standard deviation values indicated good repeatability of the analytical procedure. The developed method was successfully applied to commercial tablet preparations and produced assay results within acceptable pharmaceutical limits. The findings indicate that the proposed UV spectrophotometric absorption factor method is appropriate for routine quality control analysis of combined thiamine and pyridoxine tablet formulations.

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